ERO1L Antibody

• Background:

  Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.

• NCBI Gene ID:

  30001

• Swiss Prot:

  Q96HE7

• Accession Number:

  Q96HE7

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ERO1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-379 amino acids from the C-terminal region of human ERO1L.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  54 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  ERO1L

• NCBI Official Name:

  ERO1-like protein alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Inaba, K., et al. EMBO J. 29 (19) :3330-3343 (2010)

• Background Reference 02:

  Appenzeller-Herzog, C., et al. EMBO J. 29 (19) :3318-3329 (2010)

• Background Reference 03:

  Swiatkowska, M., et al. J. Biol. Chem. 285 (39) :29874-29883 (2010)

• Background Reference 04:

  Chambers, J.E., et al. J. Biol. Chem. 285 (38) :29200-29207 (2010)

• Other Product Names:

  ERO1-like protein alpha, ERO1-L, ERO1-L-alpha, 184-, Endoplasmic oxidoreductin-1-like protein, Oxidoreductin-1-L-alpha, ERO1L

• Tested Applications:

  WB, IHC-P

• Protein ID:

  50400608

• Physical Properties:

  Liquid