Fas Antibody [APO-1-3] (preservative free)

• Background:

  Fas (CD95) is a member of the death receptor (DR) family, a subfamily of the tumor necrosis factor receptor superfamily. The formation of the Fas death-inducing signaling complex (DISC) is the initial step of Fas signaling. Activation of procaspase-8 at the DISC leads to the induction of DR-mediated apoptosis. Stimulation of Fas has been also reported to trigger non-apoptotic pathways. It has been shown that membrane-bound FasL is essential for the cytotoxic activity, whereas soluble FasL appears to promote autoimmunity and tumorigenesis via induction of non-apoptotic pathways, in particular NF-kappaB.

• NCBI Gene ID:

  355

• Swiss Prot:

  P25445

• Accession Number:

  4507583

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human Fas.

• Clonality:

  Monoclonal

• Clone:

  APO-1-3

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies, Functional Antibodies

• Field of Research:

  Apoptosis, Cancer

• Purification:

  >95% (SDS-PAGE)

• Concentration:

  1 mg/ml

• Buffer:

  Liquid. In PBS. Preservative free formulation.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 1 year after receipt when stored at -20˚ C.

• Fragment:

  IgG3

• Specificity:

  Recognizes human Fas.

• Applications Notes:

  ELISA: (direct or indirect: 1:2,000-1:10,000) . Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.

• Symbol:

  FAS

• NCBI Official Name:

  Fas cell surface death receptor

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  CD95; APO-1; TNFRSF6; Tumor Necrosis Factor Receptor Superfamily Member 6; Apoptosis-mediating Surface Antigen FAS

• Tested Applications:

  Flow, Functional Assays, IP, WB

• Protein ID:

  NP_000034.1

• Physical Properties:

  Liquid