Multi-Cytokeratin Antibody [SPM583]

• Background:

  This mAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

• NCBI Gene ID:

  3.85138523853385e+31

• Swiss Prot:

  P19013, P13647, P02538, P04259, P48668, P05787, P13645, P13646, P05783

• Host:

  Mouse

• Reactivity:

  Human, Bovine, Rat, Mouse, Guinea Pig, Frog, Goat, Marmoset, Pig

• Immunogen:

  A keratin-enriched preparation from cultured human A431 cells was used as the immunogen for this anti-Multi-Cytokeratin antibody.

• Clonality:

  Monoclonal

• Clone:

  SPM583

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  KRT4, KRT5, KRT6A, KRT6B, KRT8, KRT10, KRT13, KRT18

• NCBI Official Name:

  Keratin 4, keratin 5, keratin 6A, keratin 6B, keratin 8, keratin 10, keratin 13, keratin 18

• NCBI Organism:

  Homo sapiens

• Tested Applications:

  Flow, IF, WB, IHC

• Physical Properties:

  Liquid