Bcl-X Antibody [SPM165]

• Background:

  Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.

• NCBI Gene ID:

  598

• Swiss Prot:

  Q07817

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat, Pig

• Immunogen:

  Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this anti-Bcl-X antibody.

• Clonality:

  Monoclonal

• Clone:

  SPM165

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG2a

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  BCL2L1

• NCBI Official Name:

  BCL2-like 1

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Bcl-xL Antibody: BCLX, BCL2L, BCLXL, BCLXS, Bcl-X, bcl-xL, bcl-xS, PPP1R52, BCL-XL/S, BCLX, Bcl-2-like protein 1, Apoptosis regulator Bcl-X, Bcl2-L-1

• Tested Applications:

  Flow, IF, WB, IHC

• Physical Properties:

  Liquid