CCND1 Antibody [SPM587]

• Background:

  Cyclin D1 (CCND1), one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of Cyclin D1. This antibody is useful in identifying mantle cell lymphomas (CCND1+) from CLL/SLL and follicular lymphomas (CCND1-) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express CCND1.

• NCBI Gene ID:

  595

• Swiss Prot:

  P24385

• Host:

  Mouse

• Reactivity:

  Human, Monkey, Mouse, Rat

• Immunogen:

  Recombinant full length human protein was used as the immunogen for this CCND1 antibody.

• Clonality:

  Monoclonal

• Clone:

  SPM587

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG2a, kappa

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  CCND1

• NCBI Official Name:

  Cyclin D1

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1

• Tested Applications:

  Flow, IF, WB, IHC

• Physical Properties:

  Liquid