APEX1 Antibody

• Background:

  Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells.

• NCBI Gene ID:

  11792

• Swiss Prot:

  Q544Z7

• Accession Number:

  NP_033817

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse APEX1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  SP2/0 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store APEX1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  35 kDa

• Applications Notes:

  APEX1 antibody can be used for detection of APEX1 by ELISA at 1:62500. APEX1 antibody can be used for detection of APEX1 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  APEX1

• NCBI Official Name:

  Apurinic/apyrimidinic endonuclease 1

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Izumi, T., et al., (2005) Proc. Natl. Acad. Sci. U.S.A. 102 (16), 5739-5743.

• Other Product Names:

  APEX1, APE, Apex, HAP1, Ref-1

• Tested Applications:

  ELISA, WB

• Protein ID:

  6753086

• Physical Properties:

  Liquid