SKA2 Antibody

• Background:

  SKA2 Antibody: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.

• NCBI Gene ID:

  348235

• Swiss Prot:

  Q8WVK7

• Accession Number:

  NP_872426

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  SKA2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1282 - 3T3 (NIH) Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (93%), Rat: (80%)

• Buffer:

  SKA2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  SKA2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  SKA2

• NCBI Official Name:

  Spindle and kinetochore associated complex subunit 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Cleveland DW, Mao Y, and Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell2003; 112:407-21.

• Background Reference 02:

  Hanisch A, Sillje HHW, and Nigg EA. Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska EMBO J.2006; 25:5504-15.

• Background Reference 03:

  Gaitanos TN, Santamaria A, Jeyaprakash AA, et al. Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf EMBO J.2009; 28:1442-52.

• Background Reference 04:

  Rice L, Water CE, Eccles J, et al. Identification and functional analysis of SKA2 interaction with the glucocorticoid receptor. J. Endocrinol.2008; 198:499-509.

• Other Product Names:

  SKA2 Antibody: FAM33A, FAM33A, Spindle and kinetochore-associated protein 2, Protein FAM33A

• Tested Applications:

  ELISA, WB

• Protein ID:

  32699054

• Physical Properties:

  Liquid