LSD1 Antibody

• Background:

  LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.

• NCBI Gene ID:

  23028

• Swiss Prot:

  O60341

• Accession Number:

  NP_001009999

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  3763

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar LSD1 expression profile in human and mouse cell lines detected by two independent anti-LSD1 antibodies that recognize different epitopes, 3761 against N-terminus domain and 3763 against central domain. LSD1 proteins are detected in the most tested cell lines that include mouse (3T3/NIH) and rat cell lines (YB2/0) at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3761 3763

• Field of Research:

  Homeostasis, Apoptosis

• Purification:

  LSD1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1286 - P815 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  LSD1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  LSD1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human LSD1 has 2 isoforms, including isoform 1 (852aa, 93kD) and isoform 2 (876aa, 95kD) . Mouse LSD1 has one isoform (853aa, 93kD) and Rat LSD1 also has one isoform (872aa, 95kD) . 3763 can detect all isoforms of human, mouse and rat.

• Symbol:

  KDM1A

• Positive Control 2:

  Cat. No. 1308 - Human Small Intestine Tissue Lysate

• Positive Control 3:

  Cat. No. 11-801 - Human Small Intestine Tissue Slide

• NCBI Official Name:

  Lysine demethylase 1A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shi et al. Cell 2004; 119:941-53.

• Background Reference 02:

  Kouzarides et al. Curr. Opin. Genet. Dev. 2002; 12:198-209.

• Background Reference 03:

  Shi et al. Mol. Cell 2005; 19:857-64.

• Background Reference 04:

  Nakamura et al. Mol. Cell 2002; 10:1119-28.

• Other Product Names:

  LSD1 Antibody: AOF2, KDM1, LSD1, BHC110, AOF2, KIAA0601, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  58761544

• Physical Properties:

  Liquid