XBP-1 Antibody

• Background:

  XBP-1 Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

• NCBI Gene ID:

  7494

• Swiss Prot:

  P17861

• Accession Number:

  P17861

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  3687

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  KO Validation (Figure 2) : XBP-1 expression detected by anti-XBP-1 antibodies was observed in wild type HeLa cells, but not in the XBP-1 knockout HeLa cells. KO Validation Recombinant Protein Test (Figure 3) : Anti-XBP-1 antibodies (3687) detected human XBP-1 recombinant protein at different concentrations. Recombinant Protein Test3687

• Field of Research:

  Signal Transduction, Chemokines & Cytokines

• Purification:

  XBP-1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1414 – Mouse Pancreas Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%)

• Buffer:

  XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  XBP-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human XBP-1 has two isoforms, including isoform 1 (261aa, 29kD) and isoform 2 (376aa, 40kD) . Mouse XBP-1 also has two isoforms, including isoform 1 (267aa, 29.6kD) and isoform 2 (371aa, 40kD) . And Rat XBP-1 has two isoforms as well, including isoform 1 (267aa, 30kD) and isoform 2 (371aa, 40kD) . 3687 can detect human, mouse and rat.

• Symbol:

  XBP1

• Positive Control 2:

  Cat. No. 1464 – Rat Liver Tissue Lysate

• NCBI Official Name:

  X-box binding protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yoshida et al. Cell 2001; 107:881-91.

• Background Reference 02:

  Calfon et al. Nature 2002; 415:92-6.

• Background Reference 03:

  Haze et al. Mol. Cell. Biol. 1999; 10:3787-99.

• Background Reference 04:

  Little et al. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

• Citation 01:

  PMID:19363290

• Citation 02:

  PMID: 19808675

• Other Product Names:

  XBP-1 Antibody: XBP2, TREB5, XBP-1, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5

• Tested Applications:

  ELISA, WB, ICC, IHC-P, IF

• Protein ID:

  60416406

• Physical Properties:

  Liquid