PPM1A (NM_021003) Human Recombinant Protein

• Background:

  The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

• Synonyms:

  PP2C-ALPHA; PP2CA; PP2Calpha

• Gene ID:

  5494

• UniProt:

  P35813, A0A024R6A5

• Accession Number mRNA:

  NM_021003

• Chromosomal Location:

  14q23.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  MAPK signaling pathway

• Field of Research:

  MAPK signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Phosphatase

• Molecular Weight:

  42.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PPM1A

• Species:

  Human

• Protein ID:

  NP_066283

• Overview:

  Recombinant protein of human protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform (PPM1A), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/5494

• Uniprot URL:

  https://www.uniprot.org/uniprot/P35813

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_021003

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_066283