CDK2AP1 (NM_004642) Human Recombinant Protein

• Background:

  The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

• Synonyms:

  Doc-1; DOC1; DORC1; p12DOC-1; ST19

• Gene ID:

  8099

• UniProt:

  O14519

• Accession Number mRNA:

  NM_004642

• Chromosomal Location:

  12q24.31

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  12.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CDK2AP1

• Species:

  Human

• Protein ID:

  NP_004633

• Overview:

  Recombinant protein of human cyclin-dependent kinase 2 associated protein 1 (CDK2AP1)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/8099

• Uniprot URL:

  https://www.uniprot.org/uniprot/O14519

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_004642

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_004633