ATP6V0A4 (NM_020632) Human Recombinant Protein

• Background:

  This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008]

• Synonyms:

  A4; ATP6N1B; ATP6N2; DRTA3; RDRTA2; RTA1C; RTADR; STV1; VPH1; VPP2

• Gene ID:

  50617

• UniProt:

  Q9HBG4, A0A024R791

• Accession Number mRNA:

  NM_020632

• Chromosomal Location:

  7q34

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

• Field of Research:

  Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Transmembrane

• Molecular Weight:

  96.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ATP6V0A4

• Species:

  Human

• Protein ID:

  NP_065683

• Overview:

  Recombinant protein of human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/50617

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9HBG4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_020632

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_065683