MDA5 (IFIH1) (NM_022168) Human Recombinant Protein

• Background:

  IFIH1 encodes MDA5 which is an intracellular sensor of viral RNA that triggers the innate immune response. Sensing RNA length and secondary structure, MDA5 binds dsRNA oligonucleotides with a modified DExD/H-box helicase core and a C-terminal domain, thus leading to a proinflammatory response that includes interferons. It has been shown that Coronaviruses (CoVs) as well as various other virus families, are capable of evading the MDA5-dependent interferon response, thus impeding the activation of the innate immune response to infection. MDA5 has also been shown to play an important role in enhancing natural killer cell function in malaria infection. In addition to its protective role in antiviral responses, MDA5 has been implicated in autoimmune and autoinflammatory diseases such as type 1 diabetes, systemic lupus erythematosus, and Aicardi-Goutieres syndrome[provided by RefSeq, Jul 2020]

• Synonyms:

  AGS7; Hlcd; IDDM19; MDA-5; MDA5; RLR-2; SGMRT1

• Gene ID:

  64135

• UniProt:

  Q9BYX4

• Accession Number mRNA:

  NM_022168

• Chromosomal Location:

  2q24.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  RIG-I-like receptor signaling pathway

• Field of Research:

  RIG-I-like receptor signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  2848756528842784

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  116.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IFIH1

• Species:

  Human

• Protein ID:

  NP_071451

• Overview:

  Recombinant protein of human interferon induced with helicase C domain 1 (IFIH1)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/64135

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9BYX4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_022168

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_071451