PSMF1 (NM_006814) Human Recombinant Protein

• Background:

  The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

• Synonyms:

  PI31

• Gene ID:

  9491

• UniProt:

  Q92530, A0A140VJT2

• Accession Number mRNA:

  NM_006814

• Chromosomal Location:

  20p13

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Proteasome

• Field of Research:

  Proteasome

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  29.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PSMF1

• Species:

  Human

• Protein ID:

  NP_006805

• Overview:

  Recombinant protein of human proteasome (prosome, macropain) inhibitor subunit 1 (PI31) (PSMF1), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/9491

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q92530

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_006814

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_006805