P16INK4A (CDKN2A) (NM_058195) Human Recombinant Protein

• Background:

  This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]

• Synonyms:

  ARF; CDK4I; CDKN2; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16

• Gene ID:

  1029

• UniProt:

  Q8N726

• Accession Number mRNA:

  NM_058195

• Chromosomal Location:

  9p21.3

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer

• Field of Research:

  Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris, 8M Urea, pH8.0.

• Function:

  Druggable Genome

• Molecular Weight:

  13.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CDKN2A

• Species:

  Human

• Protein ID:

  NP_478102

• Overview:

  Purified recombinant protein of Human cyclin-depEndent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) (CDKN2A), transcript variant 4, Met42-End, with N-terminal His tag, expressed in E.coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/1029

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8N726

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_058195

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_478102