Mkln1 (NM_013791) Mouse Recombinant Protein

• Background:

  Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (By similarity) . Required for internalization of the GABA receptor GABRA1 from the cell membrane via endosomes and subsequent GABRA1 degradation (PubMed:21482357) . Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1 (PubMed:9724633, PubMed:18710924) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  A130067F06Rik; AU015903; BB154892

• Gene ID:

  27418

• UniProt:

  O89050, Q3UWT3

• Accession Number mRNA:

  NM_013791

• Chromosomal Location:

  6 A3.3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  84.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Mkln1

• Species:

  Mouse

• Protein ID:

  NP_038819

• Overview:

  Purified recombinant protein of Mouse muskelin 1, intracellular mediator containing kelch motifs (Mkln1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/27418

• Uniprot URL:

  https://www.uniprot.org/uniprot/O89050

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_013791

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_038819