Ercc6 (NM_001081221) Mouse Recombinant Protein

• Background:

  Essential factor involved in transcription-coupled nucleotide excision repair which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (By similarity) . Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA (By similarity) . It is required for transcription-coupled repair complex formation. It recruits the CSA complex (DCX (ERCC8) complex), nucleotide excision repair proteins and EP300 to the sites of RNA polymerase II-blocking lesions (By similarity) . Plays an important role in regulating the choice of the DNA double-strand breaks (DSBs) repair pathway and G2/M checkpoint activation; DNA-dependent ATPase activity is essential for this function (By similarity) . Regulates the DNA repair pathway choice by inhibiting non-homologous end joining (NHEJ), thereby promoting the homologous recombination (HR) -mediated repair of DSBs during the S/G2 phases of the cell cycle (By similarity) . Mediates the activation of the ATM- and CHEK2-dependent DNA damage responses thus preventing the premature exit from the G2/M checkpoint (By similarity) . Acts as a chromatin remodeler at DSBs; DNA-dependent ATPase-dependent activity is essential for this function (By similarity) . Remodels chromatin by evicting histones from chromatin flanking DSBs, limiting RIF1 accumulation at DSBs thereby promoting BRCA1-mediated HR (By similarity) . Required for stable recruitment of ELOA and CUL5 to DNA damage sites (By similarity) . Involved in UV-induced translocation of ERCC8 to the nuclear matrix (By similarity) . Essential for neuronal differentiation and neuritogenesis; regulates transcription and chromatin remodeling activities required during neurogenesis (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  4732403I04; C130058G22Rik; CSB

• Gene ID:

  319955

• UniProt:

  F8VPZ5

• Accession Number mRNA:

  NM_001081221

• Chromosomal Location:

  14 B

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  166.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ercc6

• Species:

  Mouse

• Protein ID:

  NP_001074690

• Overview:

  Purified recombinant protein of Mouse excision repair cross-complementing rodent repair deficiency, complementation group 6 (Ercc6), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/319955

• Uniprot URL:

  https://www.uniprot.org/uniprot/F8VPZ5

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001081221

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001074690