Pou4f1 (NM_011143) Mouse Recombinant Protein

• Background:

  Multifunctional transcription factor with different regions mediating its different effects (PubMed:10640682, PubMed:8621561, PubMed:9694219, PubMed:9722627) . Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes (PubMed:8621561, PubMed:17668438) . Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor (PubMed:12934100) . Ativates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain) (PubMed:9722627) . Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins (PubMed:8972215) . Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity (PubMed:9448000) . May positively regulate POU4F2 and POU4F3 (PubMed:8876243) . Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord (PubMed:22326227) . Plays a role in TNFSF11-mediated terminal osteoclast differentiation (PubMed:17668438) . Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site (PubMed:12441296) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Brn-3; Brn-3.0; Brn3; Brn3.0; Brn3a; E130119J07Rik

• Gene ID:

  18996

• UniProt:

  P17208

• Accession Number mRNA:

  NM_011143

• Chromosomal Location:

  14 E2.3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  43.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Pou4f1

• Species:

  Mouse

• Protein ID:

  NP_035273

• Overview:

  Purified recombinant protein of Mouse POU domain, class 4, transcription factor 1 (Pou4f1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/18996

• Uniprot URL:

  https://www.uniprot.org/uniprot/P17208

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_011143

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_035273