Aminoacylase 1 (ACY1) (NM_000666) Human Recombinant Protein

• Background:

  This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010]

• Synonyms:

  ACY-1; ACY1D; HEL-S-5

• Gene ID:

  95

• UniProt:

  Q03154, V9HWA0

• Accession Number mRNA:

  NM_000666

• Chromosomal Location:

  3p21.2

• Expression System:

  Sf9

• Tag:

  C-DDK

• Related Pathways:

  Arginine and proline metabolism, Metabolic pathways

• Field of Research:

  Arginine and proline metabolism, Metabolic pathways

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris-HCl, pH8.0, 100mM glycine, 10% glycerol.

• Function:

  Protease

• Molecular Weight:

  45.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ACY1

• Species:

  Human

• Protein ID:

  NP_000657

• Overview:

  Purified recombinant protein of Human aminoacylase 1 (ACY1), transcript variant 1, full length, with C-terminal DDK tag, expressed in sf9, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/95

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q03154

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000666

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000657