NCF4 (NM_000631) Human Recombinant Protein

• Background:

  The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI (3) kinase, which suggests its role in PI (3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

• Synonyms:

  CGD3; NCF; P40PHOX; SH3PXD4

• Gene ID:

  4689

• UniProt:

  Q15080

• Accession Number mRNA:

  NM_000631

• Chromosomal Location:

  22q12.3

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Leukocyte transendothelial migration

• Field of Research:

  Leukocyte transendothelial migration

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl.

• Molecular Weight:

  38.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  NCF4

• Species:

  Human

• Protein ID:

  NP_000622

• Overview:

  Purified recombinant protein of Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/4689

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q15080

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000631

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000622