PNPLA1 (NM_173676) Human Recombinant Protein

• Background:

  The protein encoded by this gene belongs to the patatin-like phospholipase (PNPLA) family, which is characterized by the presence of a highly conserved patatin domain. PNPLA family members have diverse lipolytic and acyltransferase activities, and are key elements in lipid metabolism. While other members of this family have been well characterized, the function of this gene remained an enigma. However, recent studies show that this gene is expressed in the skin epidermal keratinocytes, and has a role in glycerophospholipid metabolism in the cutaneous barrier. Consistent with these observations, mutations in this gene are associated with ichthyosis in human (autosomal recessive congenital ichthyoses, ARCI) and dog. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

• Synonyms:

  ARCI10; dJ50J22.1

• Gene ID:

  285848

• UniProt:

  Q8N8W4

• Accession Number mRNA:

  NM_173676

• Chromosomal Location:

  6p21.31

• Expression System:

  E. coli

• Tag:

  N-GST and C-His

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1% Sarkosyl.

• Molecular Weight:

  75.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PNPLA1

• Species:

  Human

• Protein ID:

  NP_775947

• Overview:

  Purified recombinant protein of Human patatin-like phospholipase domain containing 1 (PNPLA1), transcript variant 1, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/285848

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8N8W4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_173676

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_775947