TEF (NM_003216) Human Recombinant Protein

• Background:

  This gene encodes a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB) . Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP) ; VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

• Synonyms:

  KIAA1655

• Gene ID:

  7008

• UniProt:

  Q10587

• Accession Number mRNA:

  NM_003216

• Chromosomal Location:

  22q13.2

• Expression System:

  E. coli

• Tag:

  N-His

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1% Sarkosyl.

• Function:

  Transcription Factors

• Molecular Weight:

  33.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TEF

• Species:

  Human

• Protein ID:

  NP_003207

• Overview:

  Purified recombinant protein of Human thyrotrophic embryonic factor (TEF), transcript variant 1, full length, with N-terminal His tag, expressed in E. coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/7008

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q10587

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_003216

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_003207