Influenza A H10N9 (A/duck/Hong Kong/562/1979) Hemagglutinin / HA Protein (His Tag)

• Background:

  The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

• Gene Name:

  Harvey rat sarcoma viral oncogene homolog

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to agglutinate guinea pig red blood cells.HA titer is 0.04-0.2μg/mL for 1%GRBC

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Viral Proteins, A/duck/HongKong/562/1979, H10N9

• Molecular Weight:

  The recombinant hemagglutinin of Influenza A virus (A/duck/Hong Kong/562/1979 (H10N9) ) consists 519 amino acids and predicts a molecular mass of 57.8 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  HA

• Species:

  H10N9

• Protein ID:

  ABI84469.1

• Overview:

  A DNA sequence encoding the Influenza A virus (A/duck/Hong Kong/562/1979 (H10N9) ) hemagglutinin (ABI84469.1) (Met1-Asp524), termed as HA, was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Asp524

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/ABI84469.1