Angiopoietin-2 Protein, Canine, Recombinant (His Tag)

• Background:

  Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.

• Gene Name:

  Angiopoietin 2

• Expression System:

  HEK293 Cells

• Tag:

  N-His

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  1.Measured by its binding ability in a functional ELISA. 2.Immobilized canine ANGPT2 (Cat:PO55336M1) at 10μg/mL (100μL/well) can bind human Tie2-Fch (Cat:PH52096M4), the ECof human Tie2-Fch is 10-50 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Diagnostic proteins, Growth Factor proteins, Cytokine Proteins, Cell Therapy proteins, Biomarker Proteins, Biological Target proteins

• Molecular Weight:

  The recombinant canine ANGPT2 consists 241 amino acids and predicts a molecular mass of 27.8 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ANGPT2

• Species:

  Canine

• Protein ID:

  NP_001041591.1

• Overview:

  A DNA sequence encoding the canine ANGPT2 (NP_001041591.1) (Lys274-Phe495) was expressed with a polyhistidine tag at the N-terminus.

• AA Sequence:

  Lys274-Phe495

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001041591.1