Prostaglandin D2 Synthase Protein, Mouse, Recombinant (His Tag)

• Background:

  PTGDS, also known as L-PGDS, belongs to the calycin superfamily, lipocalin family. Lipocalins share limited regions of sequence homology and a common tertiary structure architecture. They transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids. PTGDS is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of PGH2 to PGD2. It is involved in smooth muscle contraction/relaxation and a variety of central nervous system functions. PTGDS may have an anti-apoptotic role in oligodendrocytes. It binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.

• Synonyms:

  21kDa Protein, Mouse; L-PGDS Protein, Mouse; PGD2 Protein, Mouse; PGDS Protein, Mouse; PGDS2 Protein, Mouse; Ptgs3 Protein, Mouse

• Gene Name:

  Prostaglandin D2 synthase 21kDa (brain)

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Neurotransmitter Associated Enzymes

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant mouse Ptgds consists 176 amino acids and predicts a molecular mass of 19.9 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PTGDS

• Species:

  Mouse

• Protein ID:

  NP_032989.2

• Overview:

  A DNA sequence encoding the mouse Ptgds (NP_032989.2) (Met1-Glu189) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Glu189

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_032989.2