MEK1 Protein, Mouse, Recombinant (His & GST Tag)

• Background:

  MEK1, also known as MAP2K1 and MKK1, is a member of the dual-specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in the brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation, and development. Binding extracellular ligands such as growth factors, cytokines, and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause Cardiofaciocutaneous Syndrome.

• Synonyms:

  MAPKK1 Protein, Mouse; Mek1 Protein, Mouse; MEKK1 Protein, Mouse; Prkmk1 Protein, Mouse

• Gene Name:

  Mitogen-activated protein kinase kinase 1

• UniProt:

  P31938

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-GST & His

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  No Kinase Activity

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Kinase proteins, Cancer proteins, Enzyme Proteins

• Molecular Weight:

  The recombinant mouse MAP2K1/GST chimera consists of 630 amino acids and has a calculated molecular mass of 71.3 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  MAP2K1

• Species:

  Mouse

• Overview:

  A DNA sequence encoding the mouse MAP2K1 (P31938) (Met 1-Ile 393) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P31938

• AA Sequence:

  Met1-Ile393