IL1RAPL1 Protein, Human, Recombinant (His Tag)

• Background:

  Interleukin-1 receptor accessory protein-like 1 (IL1RAPL1) is a member of the interleukin-1 receptor family. The protein structurally comprises three extracellular immunoglobulin domains, which presumably mediate binding of an as yet unidentified ligand, a transmembrane region, and an intracellular domain, which is likely to enable signaling via the NFkB pathway. The means of signaling is almost certain to be identical to that used by the IL1R family and the more distally related Toll protein. L1RAPL1 protein physically interacts via its 150 aa C-terminal domain with neuronal calcium sensor-1 (NCS-1), a protein widely expressed in neurons and the related chromaffin and PC12 cells. IL1RAPL1 is an integral membrane protein responsible for a nonsyndromic form of mental retardation (MR) . It is suggested to affect the human cognitive ability to some extent, especially the memory and concentration capability.

• Synonyms:

  IL1R8 Protein, Human; IL1RAPL Protein, Human; MRX10 Protein, Human; MRX21 Protein, Human; MRX34 Protein, Human; OPHN4 Protein, Human; TIGIRR-2 Protein, Human

• Gene Name:

  Interleukin 1 receptor accessory protein-like 1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Receptor Proteins, Cytokine Proteins, Interleukin proteins

• Molecular Weight:

  The recombinant human IL1R8 consists of 347 amino acids and has a predicted molecular mass of 40 kDa. As a result of glycosylation, the apparent molecular mass of rh IL1R8 is approximately 50-55 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL1RAPL1

• Species:

  Human

• Protein ID:

  NP_055086.1

• Overview:

  A DNA sequence encoding the human IL1R8 (NP_055086.1) extracellular domain (Met 1-Leu 354) was expressed with a C-terminal polyhistidine tag.

• AA Sequence:

  Met1-Leu354

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_055086.1