PTPMT1 Protein, Human, Recombinant (His Tag)

• Background:

  PTPMT1 (PTP localized to the Mitochondrion 1) is a member of the protein tyrosine phosphatase superfamily that is localized exclusively to the mitochondrion. It has been recently reported that PTPMT1 dephosphorylates phosphatidylglycerol phosphate, an essential intermediate of cardiolipin biosynthesis. PTPMT1 deficiency in mouse embryonic fibroblasts compromises mitochondrial respiration and results in abnormal mitochondrial morphology. Lipid analysis of PTPMT1-deficient fibroblasts reveals an accumulation of PGP along with a concomitant decrease in phosphatidylglycerol. Modulation of mitochondrial ATP synthesis by PTPMT1 suggests a novel approach for the treatment of pancreatic cancers, which represent some of the deadliest forms of human tumors. The gluttony of cancer cells for energy is well established, and with the development of a modulator of expression, one may hope that we could also achieve the synthetic induction of PTPMT1 expression. It would then be expected that this effect would attenuate, if not abolish, the growth of pancreas-derived tumor cells and support the establishment of a novel regimen for pancreatic cancers.

• Synonyms:

  DUSP23 Protein, Human; FLJ46081 Protein, Human; MOSP Protein, Human; PLIP Protein, Human; PNAS-129 Protein, Human

• Gene Name:

  Protein tyrosine phosphatase, mitochondrial 1

• UniProt:

  Q8WUK0-1

• Expression System:

  E. coli

• Tag:

  N-His

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 94 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to cleave pNPP.The specific activity is >200 pmoles/min/μg.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, 10% glycerol, 1mM DTT, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins

• Molecular Weight:

  The recombinant human PTPMT1 consisting of 190 amino acids and has a calculated molecular mass of 21.7 kDa. It migrates as an 20 kDa band in SDS-PAGE under reducing conditions as predicted.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PTPMT1

• Species:

  Human

• Overview:

  A DNA sequence encoding the human PTPMT1 isoform 1 (Q8WUK0-1) (Lys 28-Thr 201) was expressed, with a polyhistidine tag at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8WUK0-1

• AA Sequence:

  Lys28-Thr201