MKK6 Protein, Human, Recombinant

• Background:

  Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6, and MKK6, is a protein that belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and to a lesser extent in liver or pancreas.

• Synonyms:

  MAPKK6 Protein, Human; MEK6 Protein, Human; MKK6 Protein, Human; PRKMK6 Protein, Human; SAPKK-3 Protein, Human; SAPKK3 Protein, Human

• Gene Name:

  Mitogen-activated protein kinase kinase 6

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  No tag

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Kinase activity untested

• Form:

  Liquid

• Buffer:

  Supplied as sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Kinase proteins, Enzyme Proteins

• Molecular Weight:

  The secreted recombinant human MKK6 consists of 336 amino acids and predicts a molecular mass of 37.6 KDa. The apparent molecular mass of the protein is approximately 37 KDa in SDS-PAGE under reducing conditions due to glycosylation.

• Storage Conditions:

  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  MAP2K6

• Species:

  Human

• Protein ID:

  NP_002749.2

• Overview:

  A DNA sequence encoding the human MKK6 (NP_002749.2) (Met 1-Asp334) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

• AA Sequence:

  Met1-Asp334

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_002749.2