MKK6 Protein, Human, Recombinant (207 Ser/Asp, 211 Thr/Asp, His & GST Tag)

• Background:

  Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6, and MKK6, is a protein that belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and to a lesser extent in liver or pancreas.

• Synonyms:

  MAPKK6 Protein, Human; MEK6 Protein, Human; MKK6 Protein, Human; PRKMK6 Protein, Human; SAPKK-3 Protein, Human; SAPKK3 Protein, Human

• Gene Name:

  Mitogen-activated protein kinase kinase 6

• UniProt:

  P52564-1

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  N-GST & His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 88 % as determined by SDS-PAGE.

• Activity:

  The specific activity was determined to be > 200 nmol/min/mg using inactive MAPK14 as substrate.

• Form:

  Liquid

• Buffer:

  Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Kinase proteins, Enzyme Proteins

• Molecular Weight:

  The recombinant human MAP2K6 (S207D&T211D) /GST chimera consists of 571 amino acids and has a calculated molecular mass of 65.3 KDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  MAP2K6

• Species:

  Human

• Overview:

  A DNA sequence encoding the human MAP2K6 isoform 1 (P52564-1) (Met 1-Asp 334, Ser 207/Asp, Thr 211/Asp) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P52564-1

• AA Sequence:

  Met1-Asp334, Ser207/Asp,211Thr/Asp