IP-10/CXCL10 Protein, Human, Recombinant

• Background:

  (C-X-C motif) ligand (CXCL) 10 (CXCL10) belongs to the ELR (-) CXC subfamily chemokine. CXCL10/IP-10 exerts its function through binding to chemokine (C-X-C motif) receptor 3 (CXCR3), a seven trans-membrane receptor coupled to G proteins. CXCL10/IP-10 and its receptor, CXCR3, appear to contribute to the pathogenesis of many autoimmune diseases, organ specific (such as type 1 diabetes, autoimmune thyroiditis, Graves' disease and ophthalmopathy), or systemic (such as rheumatoid arthritis, psoriatic arthritis, systemic lupus erythematosus, mixed cryoglobulinemia, Sj gren syndrome, or systemic sclerosis) . CXCL10/IP-10 is secreted by several cell types including endothelial cells, fibroblasts, keratinocytes, thyrocytes, preadipocytes, etc. Determination of high level of CXCL10/IP-10 in peripheral fluids is therefore a marker of host immune response.

• Synonyms:

  C7 Protein, Human; crg-2 Protein, Human; gIP-10 Protein, Human; IFI10 Protein, Human; INP10 Protein, Human; IP-10 Protein, Human; mob-1 Protein, Human; SCYB10 Protein, Human

• Gene Name:

  Chemokine (C-X-C motif) ligand 10

• Expression System:

  E. coli

• Tag:

  No tag

• Field of Research:

  Neuroinflammation

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Immunotherapy proteins, Biological Target proteins

• Molecular Weight:

  The recombinant human CXCL10 consists of 78 amino acids and predicts a molecular mass of 8.8 KDa. It migrates as an approximately 9 KDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CXCL10

• Species:

  Human

• Protein ID:

  NP_001556.2

• Overview:

  A DNA sequence encoding the mature form of human CXCL10 (NP_001556.2) (Val22-Pro98) was expressed, with a N-terminal Met.

• AA Sequence:

  Val22-Pro98

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001556.2