S100A10 Protein, Human, Recombinant (His Tag)

• Background:

  S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S1 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A10, also known as Calpactin I light chain, Cellular ligand of annexin II, S100 calcium-binding protein A10, p10 protein, p11, ANX2LG and S100A10, is a member of the S100 family of small, dimeric EF hand-type Ca (2+) -binding proteins that generally modulate cellular target proteins in response to intracellular Ca (2+) signals. In contrast to all other S100 proteins, S100A10 is Ca (2+) insensitive because of amino acid replacements in its Ca (2+) -binding loops that lock the protein in a permanently active state. S100A10 forms a heterotetramer with annexin IIH and promotes carcinoma invasion and metastasis by plasminogen activation. S100A10 and annexin II contribute to the aggressive characteristics of anaplastic carcinoma, while playing a constitutive role in papillary carcinoma. S100A10 induces the dimerization of ANXA2 / p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target of tyrosine-specific kinase. S100A10 functions as a linker tethering certain transmembrane proteins to annexin A2 thereby assisting their traffic to the plasma membrane and/or their firm anchorage at certain membrane sites.

• Synonyms:

  42C Protein, Human; ANX2L Protein, Human; ANX2LG Protein, Human; CAL1L Protein, Human; Ca[1] Protein, Human; CLP11 Protein, Human; GP11 Protein, Human; MGC111133 Protein, Human; p10 Protein, Human; P11 Protein, Human

• Gene Name:

  S100 calcium binding protein A10

• Expression System:

  E. coli

• Tag:

  N-His

• Field of Research:

  Calcium-binding Proteins and Related Molecules

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM Tris, pH 8.5, 10% glycerol Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant human S100A10 consisting of 107 amino acids and migrates as an approximately 12.6 kDa band in SDS-PAGE under reducing conditions as predicted.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  S100A10

• Species:

  Human

• Protein ID:

  NP_002957.1

• Overview:

  A DNA sequence encoding the human S100A10 (NP_002957.1) (Pro 2-Lys 97) was expressed, with a polyhistidine tag at the N-terminus.

• AA Sequence:

  Pro2-Lys97

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_002957.1