FLT1 Protein, Human, Recombinant (His Tag)

• Background:

  Vascular endothelial growth factor receptor 1, also known as VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor and FLT1, is a single-pass type I membrane protein and secreted protein which belongs to theprotein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 / FLT1 contains sevenIg-like C2-type (immunoglobulin-like) domains and oneprotein kinase domain. VEGFR-1 / FLT1 is expressed mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR-1 / FLT1 is not expressed in tumor cell lines. VEGFR-1 / FLT1 is an essential receptor tyrosine kinase that regulates mammalian vascular development and embryogenesis. EGF-induced angiogenesis requires inverse regulation of VEGFR-1 and VEGFR-2 in tumor-associated endothelial cells. VEGFR-1 / FLT1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.

• Synonyms:

  FLT Protein, Human; FLT-1 Protein, Human; VEGFR-1 Protein, Human; VEGFR1 Protein, Human

• Gene Name:

  Fms-related tyrosine kinase 1

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) . (Conn, G. et al. ,1990 , Proc. Natl. Acad. Sci. USA 87:1323.) The EDfor this effect is typically 10-40 ng/mL in the presence of 10 ng/mL human VEGF165.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins, Diagnostic proteins, Kinase proteins, Growth Factor proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins

• Molecular Weight:

  The recombinant human FLT1 consists 313 amino acids and predicts a molecular mass of 35.6 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FLT1

• Species:

  Human

• Protein ID:

  NP_002010.2

• Overview:

  A DNA sequence encoding the human FLT1 (NP_002010.2) (Met1-Ile328) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Ile328

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_002010.2