CDKN2A Antibody: Biotin (OAPB00526-Biotin)

• Gene Name:

  Cyclin-dependent kinase inhibitor 2A

• Gene Aliases:

  ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A, P16-INK4A

• Gene ID:

  1029

• Swiss Prot:

  P42771Q8N726

• Accession Number:

  NP_000068.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  CDKN2A antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human CDKN2A.

• Target:

  The CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16 (INK4A) and p14 (ARF) . This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. Conversely, transfection of CDKN2A suppressed the growth of two independent mesothelioma cell lines, suggesting that inactivation of the CDKN2 gene is an essential step in the etiology of malignant mesotheliomas. CDKN2A induces a G1 cell cycle arrest by inhibiting the phosphorylation of the Rb protein by the cyclin-dependent kinases CDK4 and CDK6. CDKN2A is expressed as at least three distinct isoforms.

• Clonality:

  Polyclonal

• Conjugation:

  Biotin

• Type:

  Polyclonal Antibody

• Applications:

  ELISA, WB, IHC-P, IF

• Purification:

  Affinity chromatography purified via peptide column

• Concentration:

  1 mg/mL

• Homology:

  Human; Mouse; Rat

• Format:

  Liquid. Purified antibody supplied in 1x PBS buffer.

• Reconstitution:

  All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

• Shipping Conditions:

  Wet Ice

• Fragment:

  IgG

• Applications Notes:

  CDKN2A antibody can be used for detection of CDKN2A by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.

• NCBI Gene Symbol:

  CDKN2A

• Protein Name:

  Cyclin-dependent kinase inhibitor 2A