UBA3 Antibody - N-terminal region: HRP (ARP50493_P050-HRP)

• Gene Name:

  Ubiquitin-like modifier activating enzyme 3

• Gene Aliases:

  NAE2, UBE1C, hUBA3

• Gene ID:

  9039

• Swiss Prot:

  Q8TBC4

• Accession Number:

  NP_003959

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish

• Immunogen:

  The immunogen is a synthetic peptide directed towards the N terminal region of human UBA3

• Target:

  UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

• Partner Proteins:

  UBA3; UBC; IMPA2; CPNE2; NEDD8; CAMK1D; NSFL1C; DCPS; KDM1A; PAPSS1; NAE1; XPO1; PCYT2; CAPN2; AHCY; ACAT2; NT5DC1; NUDCD2; TXNDC17; WDR61; UFC1; DSTN; VCL; UBE2H; PRDX2; TWF1; UBE2M; UIMC1; CHD3

• Clonality:

  Polyclonal

• Conjugation:

  HRP: Horseradish Peroxidase

• Type:

  Polyclonal Antibody

• Applications:

  IHC, WB

• Purification:

  Affinity Purified

• Concentration:

  0.5 mg/mL

• Homology:

  Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

• Format:

  Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

• Reconstitution:

  All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

• Molecular Weight:

  52kDa

• Shipping Conditions:

  Wet Ice

• Protein Length:

  463

• NCBI Gene Symbol:

  UBA3

• Protein Name:

  NEDD8-activating enzyme E1 catalytic subunit

• Nucleotide Accession Number:

  NM_003968