Anti-Hu CD57 Biotin

• Description:

  CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.

• Certification:

  RUO

• Immunogen:

  A pool of neuroblastoma cell lines

• Target Antigen:

  CD57

• Clone:

  TB01

• Applications:

  FC (QC tested), IHC-P, ICC, IHC-F

• Concentration:

  1 mg/mL

• Format:

  Biotin

• Buffer:

  Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

• References & Citations:

  *Lim SA, Kim TJ, Lee JE, Sonn CH, Kim K, Kim J, Choi JG, Choi IK, Yun CO, Kim JH, Yee C, Kumar V, Lee KM: Ex vivo expansion of highly cytotoxic human NK cells by cocultivation with irradiated tumor cells for adoptive immunotherapy. Cancer Res. 2013 Apr 15;73 (8) :2598-607., URL: http://www.ncbi.nlm.nih.gov/pubmed/23580577, *Cunha KS, Caruso AC, Faria PA, Silva LE, Fonseca EC, Geller M, de Moura-Neto RS, Lopes VS: Evaluation of Bcl-2, Bcl-x and cleaved caspase-3 in malignant peripheral nerve sheath tumors and neurofibromas. An Acad Bras Cienc. 2013;85 (4) :1497-511., URL: http://www.ncbi.nlm.nih.gov/pubmed/24346802 , *Phadwal K, Alegre-Abarrategui J, Watson AS, Pike L, Anbalagan S, Hammond EM, Wade-Martins R, McMichael A, Klenerman P, Simon AK: A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells. Autophagy. 2012 Apr;8 (4) :677-89., URL: http://www.ncbi.nlm.nih.gov/pubmed/22302009 , *Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson MA, Kekäläinen E, Johansson H, Mjösberg J, Westgren M, Lankisch TO, Wedemeyer H, Ellis EC, Ljunggren HG, Michaëlsson J, Björkström NK: Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015 Mar 15;194 (6) :2467-71., URL: https://pubmed.ncbi.nlm.nih.gov/25672754/, *Wangerin H, Kristiansen G, Schlomm T, Stephan C, Gunia S, Zimpfer A, Weichert W, Sauter G, Erbersdobler A: CD57 expression in incidental, clinically manifest, and metastatic carcinoma of the prostate. Biomed Res Int. 2014;2014:356427., URL: https://pubmed.ncbi.nlm.nih.gov/24977150/, *Fernandez S, French MA, Price P: Immunosenescent CD57+CD4+ T-cells accumulate and contribute to interferon-γ responses in HIV patients responding stably to ART. Dis Markers. 2011;31 (6) :337-42., URL: http://www.ncbi.nlm.nih.gov/pubmed/22182806

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-5 μg/ml.

• Species:

  Human

• Isotype:

  Mouse IgM