EasyStep Human MMP9 (Matrix Metalloproteinase 9) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP9, and the Human MMP9 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MMP9. After TMB substrate solution is added, only those wells that contain Human MMP9 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

• Gene Aliases:

  GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

• UniProt:

  P14780

• Reactivity:

  Human

• Field of Research:

  Enzyme & Kinase, Tumor immunity, Infection immunity, Cardiovascular biology, Hepatology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP9, and the Human MMP9 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Human MMP9. After TMB substrate solution is added, only those wells that contain Human MMP9 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  1.5 H

• Standard:

  80 ng/mL

• Sample Type:

  Serum, plasma

• Detection Range:

  1.25 - 80 ng/mL

• Sensitivity:

  1.25 ng/mL

• Species:

  Human

• CAS Number:

  7732-18-5