Rabbit anti Human Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6)

• Background:

  EDG-6 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-6 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-6 is expressed in lymphoid and hematopoietic tissue and in the lungs. _x000B_Recently, the designation of the EDG receptors has been modified to include the ligand for the receptor. Thus, the new designation for the EDG-6 receptor is the sphingosine 1-phosphate receptor-4 (S1P-4).

• Synonyms:

  Endothelial Cell differentiation gene 6 C-terminal, Sphingosine-1-Phosphate Receptor-4 (S1P-4)

• UniProt:

  O95977

• Host:

  Rabbit

• Species Reactivity:

  Human

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Source:

  Rabbits were immunized with a synthetic peptide derived from the C-terminal of the EDG-6 receptor

• Applications:

  WB

• Field of Research:

  Signal Transduction

• Purification Method:

  Ammonium Sulfate Precipitation

• Assay Principle:

  Detects EDG6 receptors at concentration of 5-10 µg/mL by Western blot using a Human EDG6 receptor transfected cell line. Detects an approximately 45 kDa band in Cos cells transfected with full length Human EDG6. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development. Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Yamazaki, Y., et al. 'EDG-6 as a putative sphingosine 1-phosphate receptor coupling to Ca(2+) signaling pathway.' Biochem. Biophys. Res. Commun. 2000, 268, 583-589._x000D_ 2. Van Brocklyn, J.R., et al. 'Sphingosine 1-phosphate is a ligand for the G protein-coupled receptor EDG-6.' Blood 2000, 95, 2624-2629._x000D_ 3. Takuwa, Y., et al. 'Subtype-specific, differential activities of the EDG family receptors for sphingosine 1-phosphate, a novel lysophospholipid mediator.' Mol. Cell Endocrinol. 2001, 177, 3-11.

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4