Recombinant Mouse S100a9 protein (His Tag)

• Background:

  S100A9 is a calcium-and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra-and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase.

• Abbreviation:

  S100a9

• Synonyms:

  Leukocyte L1 complex heavy chain;60B8AG; CAGB; CFAG; CGLB; Calgranulin B; Calgranulin-B; Calprotectin L1H subunit; Cystic fibrosis antigen B; L1AG; LIAG; Leukocyte L1 complex heavy chain; MAC387; MIF; Migration inhibitor; Migration inhibitory factor related protein 14

• UniProt:

  P31725

• Accession Number:

  P31725

• Expression System:

  E.coli

• Tag:

  N-His & C-His

• Sequence:

  Ala2-Lys113

• Applications:

  Immunogen

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  14.3 kDa

• Observed Molecular Weight:

  15-18 kDa

• Species:

  Mouse