Recombinant Human LL-37 protein (GST tag)

• Background:

  Antimicrobial protein that is an integral component of the innate immune system. Binds to bacterial lipopolysaccharides (LPS) . Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2. Postsecretory processing generates multiple cathelicidin antimicrobial peptides with various lengths which act as a topical antimicrobial defense in sweat on skin. The unprocessed precursor form, cathelicidin antimicrobial peptide, inhibits the growth of Gram-negative E.coli and E.aerogenes with efficiencies comparable to that of the mature peptide LL-37 (in vitro) .

• Abbreviation:

  LL-37

• Synonyms:

  18 kDa cationic antimicrobial protein; CAMP; CAMP_HUMAN; CAP-18; Cathelicidin antimicrobial peptide; FALL39; HSD26; hCAP-18

• UniProt:

  P49913

• Accession Number:

  P49913

• Expression System:

  E.coli

• Tag:

  N-GST

• Sequence:

  Leu 134-Ser 170

• Field of Research:

  Microbiology

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  29.0 kDa

• Observed Molecular Weight:

  32 kDa

• Species:

  Human