Recombinant Human CXCL13/BCA-1 Protein

• Background:

  Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.

• Abbreviation:

  CXCL13, BCA-1

• Synonyms:

  Angie; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BLC; C-X-C motif chemokine 13; CXC chemokine BLC; SCYB13; Small-inducible cytokine B13

• UniProt:

  O43927

• Accession Number:

  O43927

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  Val23-Arg94

• Field of Research:

  Immunology; Cardiovascular

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  8.7 kDa

• Observed Molecular Weight:

  10 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% Tween 80.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human