Recombinant Human CDK2 Protein (E.coli, His Tag)

• Background:

  Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells.

• Abbreviation:

  CDK2

• Synonyms:

  CDK2; CDKN2; Cell Division Protein Kinase 2; Cyclin-Dependent Kinase 2; p33 Protein Kinase

• UniProt:

  P24941

• Accession Number:

  P24941

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Met 1-Leu298

• Field of Research:

  Signal Transduction; Cell biology; Neuroscience; Cancer; epigenetics and nuclear signal; metabolism

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  36.1 kDa

• Observed Molecular Weight:

  34 kDa

• Species:

  Human