TOXOPLASMA ELISA IgM CAPTURE

• Description:

  Indirect Method- The ELISA method is based upon the reaction of antibodies in the sample tested with the antigen adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. An enzyme-labelled anti-human globulin binds the antigenantibody complex in a second step. After a new washing step, bound conjugate is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution. Capture Method- The ELISA method is based upon the capture of IgM in the sample with anti-IgM antibodies adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. Then the antigen labeled with peroxidase react with the IgM captured, and the unbound is eliminated by washing; bound antigen is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution.

• Certification:

  CE0318

• Features & Benefits:

  High performance and guaranteed stability with lyophilized conjugates when necessary. Samples and controls are equally processed to compensate pipetting variability. Colour-coded plates with individual break-apart wells. Coloured, ready-to-use liquid reagents.