Human IL-10 (Interleukin 10) ELISPOT Kit

• Abbreviation:

  IL-10

• UniProt:

  P22301

• Cross Reactivity:

  This kit recognizes Human IL-10 in samples.No significant cross-reactivity or interference between Human IL-10 and analogues was observed.

• Target:

  IL10; IL10A; CSIF; TGIF

• Applications:

  ELISPOT

• Field of Research:

  Immunology

• Assay Principle:

  This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-10 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-10 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-10 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-10 antibodies will bind specifically to the IL-10 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-10 secreting cells.

• Assay Performance Time:

  3 h 30 min

• Sample Type:

  Suspension cell

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,12 months

• Species:

  Human

• Recommended Usage:

  This ELISPOT kit is designed for the detection of the frequency of human IL-10 secreting cells.