Anti-Hu Galectin-9 PE

• Description:

  Galectin-9 is a glycan-binding protein, which is expressed in three main isoforms of 49 aa, 27 aa, and 15 aa. It can be detected on the cell surface, as well as intracellularly, or in a secreted form. On the cell surface, galectin-9 plays roles in contacts with other cells and with extracellular matrix. It is expressed on multiple cell types, but mainly on Treg cells, activated Th cells and some cancers. Its secreted form acts like a cytokine with immunomodulatory and immunosuppresive functions. Massive and inadequate production of galectin-9, associated with some viral infections or cancers, can counteract immune reactions to these illnesses. High levels of galectin-9 expression lead to poor prognosis of cancer patients.

• Certification:

  RUO

• Immunogen:

  Recombinant M-type splicing variant of human galectin 9

• Target Antigen:

  Galectin-9

• Clone:

  9M1-3

• Applications:

  FC-IC (QC tested), FC

• Format:

  PE

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Chen Z, Dong D, Zhu Y, Pang N, Ding J: The role of Tim-3/Galectin-9 pathway in T-cell function and prognosis of patients with human papilloma virus-associated cervical carcinoma. FASEB J. 2021 Mar;35 (3) :e21401., URL: https://pubmed.ncbi.nlm.nih.gov/33559190/, *Motamedi M, Shahbaz S, Fu L, Dunsmore G, Xu L, Harrington R, Houston S, Elahi S: Galectin-9 expression defines a subpopulation of NK cells with impaired cytotoxic effector molecules but enhanced IFN-γ production, dichotomous to TIGIT, in HIV-1 infection. Immunohorizons. 2019 Nov 15;3 (11) :531-546. , URL: https://pubmed.ncbi.nlm.nih.gov/31732662/, *Bertino P, Premeaux TA, Fujita T, Haun BK, Marciel MP, Hoffmann FW, Garcia A, Yiang H, Pastorino S, Carbone M, Niki T, Berestecky J, Hoffmann PR, Novu LC: Targeting the C-terminus of galectin-9 induces mesothelioma apoptosis and M2 macrophage depletion. Oncoimmunology 2019 Apr 17;8 (8) :1601482., URL: https://pubmed.ncbi.nlm.nih.gov/31413910/, *Klibi J, Niki T, Riedel A, Pioche-Durieu C, Souquere S, Rubinstein E, Le Moulec S, Guigay J, Hirashima M, Guemira F, Adhikary D, Mautner J, Busson P: Blood diffusion and Th1-suppressive effects of galectin-9-containing exosomes released by Epstein-Barr virus-infected nasopharyngeal carcinoma cells. Blood 2009 Feb 26;113 (9) :1957-66. , URL: https://pubmed.ncbi.nlm.nih.gov/19005181/, *Lhuillier C, Barjon C, Baloche V, Niki T, Gelin A, Mustapha R, Claër L, Hoos S, Chiba Y, Ueno M, Hirashima M, Wei M, Morales O, Raynal B, Delhem N, Dellis O, Busson P: Characterization of neutralizing antibodies reacting with the 213-224 amino-acid segment of human galectin-9. PLoS One 2018 Sep 11;13 (9) :e0202512., URL: https://pubmed.ncbi.nlm.nih.gov/30204750/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 9M1-3 recognizes an epitope within C terminus of human galectin-9, a glycan-binding protein expressed mainly on activated Th cells and FoxP3+ Treg cells.

• Applications Notes:

  6

• Species:

  Non-human primates, Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa