Recombinant Human CD40 ligand (CD40LG), partial (Active)

• Product Name Alternative:

  (CD40-L) (T-cell antigen Gp39) (NF-related activation protein) (TRAP) (Tumor necrosis factor ligand superfamily member 5) (CD154) (sCD40L)

• Abbreviation:

  Recombinant Human CD40LG protein, partial (Active)

• Gene Name:

  CD40LG

• UniProt:

  P29965

• Expression Region:

  113-261aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

• Tag:

  N-terminal hFc-Flag-tagged

• Type:

  Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Field of Research:

  Cancer

• Relevance:

  Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973) . Costimulates T-cell proliferation and cytokine production (PubMed:8617933) . Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933) . Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973) . Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037) . Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037) . Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity) . Involved in immunoglobulin class switching

• Endotoxin:

  Less than 1.0 EU/μg as determined by LAL method.

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  ①Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody (CSB-RA156386A0HU), the EC50 is 2.353-3.232 ng/ml.

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  44.5 kDa

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Partial