Synthetic Blocker

• Description:

  Increases the Sensitivity of the Assay. This is done by stabilizing the antigenic and functional regions of the absorbed protein., Reduces Background Noise. Block buffers inhibit nonspecific binding to uncoated regions of the plate and non-specific binding sections of the absorbed proteins. This leads to lower background noise and high specific signal., Increases Efficiency and Extends the Shelf-Life of the Coated Plates. With a longer shelf-life, plates may be prepared in large batches to be used over time. Large batch sizes will increase efficiency and reduce plate-to-plate variability to increase consistency., Conserves Valuable Reagents. By promoting a higher specific signal, less protein may be needed to coat the plate and less of the detection molecules may be needed to generate a signal when the proper blocker is used.

• Label:

  ICT

• Type:

  ELISA & Assay Reagents

• Assay Protocol:

  Simply dilute Coating Buffer 1:5 (for example, add 100mL Coating Buffer to 400mL diH20 for a final volume of 500mL) , add your protein antigen or antibody, let the solution stir for 15 minutes, and pipette onto the plate.

• Concentration:

  1X

• Buffer:

  7.4 at 1X

• pH:

  7.4 at 1X

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Temperature:

  2-8°C

• Shelf Life:

  Expires two years from date of manufacture

• Target Description:

  ICT’s Synthetic Blocker is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Because Synthetic Blocker contains inert non-reactive blockers, it reduces the amount of nonspecific binding of enzyme-labeled conjugates to blocked plate surfaces. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.