HRP Conjugate Stabilizer, Mammalian, 1X

• Description:

  Preserve activity of HRP conjugates and reduce background signal in immunoassays using HRP detection systems. HRP Conjugate Stabilizer is used to preserve concentrated stock conjugates, reconstitute lyophilized conjugates, and dilute antibody-HRP conjugates to their useful working titer in ELISAs and immunology-based techniques.

• Label:

  ICT

• Type:

  ELISA & Assay Reagents

• Assay Protocol:

  1. For in vitro apoptosis detection, culture your cells to a concentration of 2-5 x 105 cells/mL., 2. Prepare experimental and control populations; concentrate cells to 5 x 105-106 cells/mL in 300 µL samples., 3. Reconstitute the FAM-OPH reagent with 50 µL DMSO to form the 150X stock concentrate (may be frozen for future use)., 4. Dilute the FAM-OPH stock concentrate with 200 µL PBS to form the 30X working solution., 5. Add FAM-OPH working solution directly to samples and controls at a ratio of 1:30. For example, add 10 µL of working solution to a 300 µL aliquot of suspension cells., 6. Incubate ~1 hour., 7. Wash and spin cells two or three times., 8. If desired, label cells with Hoechst 33342 nuclear stain, DAPI nuclear stain, or other compatible fluorescent markers., 9. If desired, fix cells with formalin-based agents., 10. Analyze data using a fluorescence microscope, plate reader, or flow cytometer.

• Concentration:

  1X

• pH:

  pH: 7.4 at 1X

• Additionnal Information:

  ELISA & Assay Reagents ; Conjugates and Stabilizers

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Temperature:

  2-8°C

• Shelf Life:

  Expires two years from date of manufacture

• Target Description:

  HRP Conjugate Stabilizer, Mammalian, 1X preserves the activity of HRP-protein conjugates during storage, reconstitution, and immunoassay use. This HRP stabilizer can be used to store concentrated stock HRP conjugates, reconstitute lyophilized conjugates, and dilute HRP conjugated antibodies to their useful working titer in ELISA tests and immunology-based techniques. HRP Conjugate Stabilizer is especially suited for ELISAs using anti-IgG HRP conjugates within traditional antigen-down or antibody sandwich formats. Additional components may be added to HRP Conjugate Stabilizer, Mammalian, 1X to optimize it for particular ELISA format compositions or for use in other immunological techniques. Antimicrobial agents are included to retard bacterial growth., , HRP Conjugate Stabilizer, Mammalian, 1X preserves the functional integrity of the horseradish peroxidase enzyme and immunoglobulin components of stock conjugates upon reconstitution or dilution to its working concentration for ELISA or other immunology-based techniques. Its proprietary formula also stabilizes the HRP conjugate during storage and enhances the bond between detection HRP conjugated antibody and target antigen or antibody., , Mammalian proteins and salt additives provide structural support to both the immunoglobulin and HRP components of the conjugate, preserving enzymatic and IgG activity and stabilizing the conjugate bond., , HRP Conjugate Stabilizer, Mammalian, 1X inhibits the HRP conjugate from binding to nonspecific serum proteins in the sample, thereby reducing conjugate bridging on the ELISA plate surface and reducing non-specific background noise.