Anti-GTA MCP | Gene Transfer Agent (GTA) major capsid protein (MCP)

• Background:

  Gene Transfer Agents (GTAs)are bacteriophage-like particles which function as a means to package and transfer genomic DNA between bacterial cells.GTA-mediated gene transfer might be an important phenomenon in natural microbial communities.

• Host:

  Rabbit

• Reactivity:

  Dinoroseobacter shibae, Rhodobacter capsulatus,Ruegeria pomeroyiDSS-3

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-conjugated conserved peptide sequence found in the Gene Transfer Agent (GTA) major capsid protein (MCP) encoded in Bacteria within the Rhodobacterales order of the class alpha-Proteobacteria includingRhodobacter sphaeroidesUniProt:Q3J3K4

• Clonality:

  Polyclonal

• Applications:

  Western blot (WB)

• Dilution:

  1 : 1000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 100 µl of sterile distilled water

• Molecular Weight:

  31.4 | 32 kDa (predicted mature capsid protein ofRhodobacter capsulatus)

• References & Citations:

  Shimizuet al. (2022) Persulfide-Responsive Transcription Factor SqrR Regulates Gene Transfer and Biofilm Formation via the Metabolic Modulation of Cyclic di-GMP in Rhodobacter capsulatus, Microorganisms 10, no. 5: 908. https://doi.org/10.3390/microorganisms10050908Koppenhoferet al. (2019). Integrated Transcriptional Regulatory Network of Quorum Sensing, Replication Control, and SOS Response in Dinoroseobacter shibae. Front. Microbiol., 12 April 2019 | https://doi.org/10.3389/fmicb.2019.00803.Tomaschet al. (2018). Packaging of Dinoroseobacter shibae DNA into Gene Transfer Agent Particles Is Not Random. Genome Biol Evol. 2018 Jan 1;10(1):359-369. doi: 10.1093/gbe/evy005.Mercerand Lang (2014). Identification of a predicted partner-switching system that affects production of the gene transfer agent RcGTA and stationary phase viability in Rhodobacter capsulatus. BMC Microbiol. 2014 Mar 19;14(1):71.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4