Human SP-D / Surfactant protein D ELISA Kit PicoKine®, Carrier-free

• Background:

  Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What’s more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

• :

  heparin or EDTA

• Description:

  Human SP-D / Surfactant protein D ELISA Kit PicoKine® (96 Tests). Quantitate Human SFTPD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	1547	1291	1390	1463	1422	94.19	6.6%
  Sample 2	3580	3482	3689	3752	3625	103.31	2.8%
  Sample 3	25269	29048	29153	26765	27558	1630.37	5.9%

  *number of samples for each test n=16.

• Synonyms:

  Pulmonary surfactant-associated protein D;PSP-D;SP-D;Collectin-7;Lung surfactant protein D;SFTPD;COLEC7, PSPD, SFTP4;

• Gene Name:

  Pulmonary surfactant-associated protein D

• Gene ID:

  6441

• UniProt:

  P35247

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: A21-F375

• Tissue Specificity:

  Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). .

• Applications:

  ELISA

• Standard:

  40 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  625 pg/ml - 40,000 pg/ml

• Sensitivity:

  <20 pg/ml

• Function:

  Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  37728 MW

• Specificity:

  Natural and recombinant human SP-D

• Subcellular Location:

  Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.

• Sequence Similarities:

  Belongs to the SFTPD family.

• Protein Name:

  Pulmonary surfactant-associated protein D